The nine circle in geometry deserve significance of correlation with circumscribed circle, inscribed circle and the Euler line for a triangle. The nine significant concyclic points on the circle recognized by the triangle include: the midpoint of each side of the triangle; the foot point of each altitude and the midpoint of the line segment from each vertex of the triangle to the orthocenter (where the three altitudes meet; these line segments lie on their respective altitudes). The present attempt is dealing with utilization of principle of “Nine point circle” in understanding the concept of relations between the concentration of substrate [S] and the velocity (v) of enzyme controlled reactions in biochemistry. The steps of the attempt include: determination of mid points of three sides of the triangular form of double reciprocal plot; foot point of each line segment forming the altitude of a triangular form of double reciprocal plot; the midpoint of the line segment from each vertex of the triangle to the point representing the orthocenter of the triangular form of double reciprocal plot and determination of center for expected circle in triangular form of double reciprocal plot. Significant feature of triangular form of double reciprocal (Lineweaver-Burk) plot in present attempt is right angled triangle. The mid points of the three sides of triangular form of double reciprocal (Lineweaver-Burk) plot are easy for location. Being a right angle triangle, the orthocenter of triangular form of double reciprocal (Lineweaver-Burk) plot in present attempt coincides with the vertex at the right angle. The right angled vertex of triangular form is serving not only as the orthocenter, but also as the foot point for the base segment and the height segment. The segment perpendicular to real form of double reciprocal (Lineweaver-Burk) plot and passing through the right angled vertex give the third altitude foot point. The mid point of base segment and height segment constitute the mid point of the segment passing from vertex to the orthocenter. The five significant points resulted on circle include: B, D, E, F and G. The x and y co-ordinates of point “B” are: 1 and (1÷Vmax) respectively. The x and y co-ordinates of point “D” are: [(1÷2)] and [(Km+2) ÷ 2Vmax)] respectively. The x and y co-ordinates of point “E” are: [(1÷1)] and [(Km+2) ÷ 2Vmax)] respectively. The x and y co-ordinates of point “F” are: (1÷2) and (1÷Vmax) respectively. The x and y co-ordinates of point “G” are: [(Vmax2 ) ÷ (Vmax2+Km2 ] and [Km(Vmax2+Km)+Vmax2 ]÷ [(Vmax 2+Km2 )Vmax] respectively. Therefore, the resulted circle is titled as, “Five Point Circle for the Triangular Form of Double Reciprocal (Lineweaver-Burk) Plot in Enzyme Biochemistry”.

Although Mongolian people use as much animal fat and salt in the food, the level of cholesterol was determined relative as normal. But recently, their life style changed by the influence of social factors and non-infections disease quite increased, including atherosclerosis. For this reason, we aimed to study the wall structure of coronary artery and to determine some biomarkers in the relatively healthy persons and coronary atherosclerotic patients’ autopsy. Twelve autopsies from 21 to 74 aged cadavers were included in this study. All specimens were processed with Haematoxylin-Eozin stained andthe changes of coronary arterial wall structure were examined by the special staining with Van-Gizon, three chrome Masson: the atherosclerotic plaques, neovascularization and fat crystals were observed in the wall of the coronary artery with atherosclerosis. By the immunohistochimical staining, the presence of vascular cell adhesion molecule (VCAM-1) was detected in the endothelial cell membrane, vascular endothelial growth factor (VEGF) was slightly immunoreacted in the nucleus, cytoplasm and of endothelial cell membrane, but PECAM-1 and LOX-1 were not detected in the wall of injured coronary artery, suggesting a role of VCAM-1 in atherosclerosis.